Many new biological products are derived from fragile organisms. Although relatively easy to separate the trick is accomplishing the separation in a gentle manner without destroying the shear sensitive cell wall membranes that isolate the complex intracellular proteins from the extracellular liquid. If this can be avoided, downstream purification of the target proteins becomes much easier.
The machine is designed for clarification duty. Especially when clarifying liquids from shear sensitive particles. Applications that requires low oxygen pick-up can also take advantage of the hermetic features this machine offers.
Unique hollow spindle design
Alfa Laval has worked for many years with machines that are bottom fed through a hollow spindle. Liquids entering the Culturefuge 100 are gradually accelerated as they move upwards through the spindle. The feed zone of the machine is completely filled with rotating liquid and there is no air/liquid interface. This smooth acceleration minimizes shear forces acting on the liquid and thus prevents cell lysis.
Another feature of the Culturefuge 100 is the completely hermetic outlet. Clarified centrates are discharged via a sealed outlet that precludes any contact with air. This is particularly desirable when the liquids are prone to foam or where the products are susceptible to oxidation.
Hygienic considerations have been of utmost importance in the design of the Culturefuge 100. It is fully steam sterilizable and has cleaning-in-place capability. Pressure vessel designs according to ASME or to PED are available. Cooling jackets surrounding the centrifuge bowl give efficient temperature control and also contribute to a low noise level. The “dosing ring” solids-ejecting system with fast and distinct discharge action ensures low product losses and drier solids. Flushing nozzles spray the outside of the centrifuge bowl, cyclone and the inside of the frame hood for maximum hygiene.
The centrifuge is mounted on a fixed base frame, which includes process piping for service liquids and process liquids entering and leaving the centrifuge. Typically, the solids phase is removed by a steam sterilizable pump. The built-in electrical system includes starter, PLC control system and pneumatic unit.
An integral motor with built-in VFD is standard and provides considerable space savings for a complete system.