Optek-Danulat’s cell growth monitors (turbidimeters) provide fast measurement of optical density in real time. No need for sampling. This eliminates the loss of volume and the errors associated with dilution. In addition, the risk of contamination is reduced.
The device continuously measures cell concentration increase. This allows the operator to determine the moment when the cell growth stops and get the best results.
The device can act as an alarming system to alert operators about abnormal variations in the cell growth cycle, which helps to prevent product loss.
Typically, turbidimeters are installed in the bioreactor. They measure the density of bacteria, yeast, fungi or mammalian cells directly. Data coming from the bioreactor is used to control the fresh medium feed rate. This ensures optimal growth rate and integrity of the final product. With the help of turbidimeters you can determine the best feed rate, the cut-off time, and optimal harvest time.
The system provides for the linearization of absorption and tuning in accordance with the methods of laboratory analysis used by the customer to determine the number of cells and the optical density. This linearized value can then be displayed in any units of measurement.
Turbidimeters can be used in systems of various scales, up to the final production bioreactor. This device is unique, since verification can be done between cycles either directly in the line during production cycle. All wetted parts can be steam sterilized.
The AS16 series offers the high-end range of optek probe sensors. A wide selection of different optical path lengths and insertion depths combined with optional calibration filters and electro-polished stainless steel meet all requirements of the biotechnology industry.
The AS56, based on the same design as the AS16 with seal-less window construction, is typically used in food and beverage applications. Limited variations allow cost effective measurement (e.g., phase separation).
A special tungsten lamp produces a constant light beam that passes through the process medium. The attenuation of the light intensity, caused by absorption and/or scattering by dissolved and undissolved substances, is detected by a sealed silicon photodiode. The AS16-N and AS56-N uses light from 730 - 970 nm to measure solids concentration independent from color or color changes (e.g., cell concentration in the culture medium).
optek AS16-N Single Channel Absorption Probe
Special optical windows are made from a single crystal sapphire, providing superior resistance to all abrasive and corrosive media. optek‘s superior manufacturing techniques allow mounting the windows without gaskets or glue for lifetime without maintenance. The appropriate choice of the optimal OPL (optical path length = distance between the windows) supports all measurement requirements, i.e., low/high measuring ranges at highest resolution.
NIST-traceable calibration accessories (AS16 only) provide absolute measurement confidence.