Fermenters 2,000L/10,000L technical data:

Connections:

1. Lid

1.1 Sight glass with light

1.2 Air inlet with sterile filter

1.3 Air outlet with sterile filter and condenser

1.4 Charge hole

1.5 Foam sensor

1.6 Additory port for foam breaker connection

1.7 Level sensor

1.8 Pressure gauge

1.9 Cleaning head

2. Upper body 

2.1 Sparger connection

2.2 Innoculation port

2.3 Safety valve

3. Lower body

Connection ports for:

3.1 Temperature Sensor

3.2 pH sensor

3.3 Dissolved oxygen sensor

3.4 Sterilizable sampler

3.5 Two spare ports

4. Bottom 

Sterilizable bottom valve

5. Jacket 

Connection of temperature control system and steam supply

6. Controllers: 

6.1 Temperature

6.2 Dissolved oxygen 0-150% +/- 1%; cascade control with agitator, airflow and pressure

6.3 pH 0-14 +/- 0.05; controlles supply of titrating agent NH3 into the air line

6.4 Pressure 1 - 3,5 bar +/- 0,1 bar

6.5 Level +/- 2%

7. Aeration system

7.1 Airflow is regulating automatically

7.2 The system provides flow rate up to 2 volumes of air per 1 volume of culture media

7.3 Flowrate meter with rotameter calibrated at 20°C, 760 mm Hg

7.4 Sterile filter, material: PTFE

7.5 Sterilization temperature at 121°C during 60 min.

7.6 Circular sparger

7.7 Aseptic return valve

7.8 Connecting of ammonia with sterilizing filter

8. Pressure Monitoring System 

8.1 Pressure is regulated automatically by the control system

8.2 Safety valve

8.3 Pressure gauge

8.4 Positioner

8.5 Pressure Sensor

8.6 Pressure control Valve

8.7 Exhaust filter

9. Sensors 

9.1 pH sensor, sterilized in place

9.2 pH sensor body

9.3 PH sensor cable

9.4 Dissolved Oxygen sensor

9.5 Dissolved oxygen sensor cable

9.6 Foam sensor

9.7 Foam sensor cable

9.8 Vessel and piping system for anti-foam agent supply

9.9 Temperature Sensor

9.10 Circulation System

Fermenters features:

• Cost-effective integration into existing infrastructure
• Powerful PLC-base automation platform with newly added functions
• Safe to use due to automatic sequences for sterilization and pressure hold test
• On-line formula calculation with possible virtual controller
• Availability of on-line customer support
• Flexible support with spare-parts over the world
• Optimized PID control loops and transmitters for the following measurements are included:
  • Vessel and double jacket temperature: Measurement, control and recording. Sterilization control via existing solenoid valves.
  • pH: Measurement control and recording. Control via acid and base pumps.
  • Dissolved oxygen: Measurement, control, and recording. Control via cascade with agitation speed or MFC Air.
  • Air and O₂ rate control via mass flow meter (if needed).
  • Agitation speed measurement control and recording utilizing frequency inverter.
  • Anti-Foam: Measurement control and recording. Control via foam pump.
  • Level: Measurement control and recording.
• 12 trends can be edited at the same time
• The database can be edited in the form of graphic or text
• It’s possible to compare several data Batches
• The database is recorded in .csv format
• The recorded files can be transferred on an electronic medium or on the network in .csv, .txt or .xls format

C-BIO2^TM control system features:

• Universal control system for fermenters of any volume.
• You can use either separate control system for each culture vessel, or one control system for several vessels.
• The system is based on SCADA controller, constructed in conformance with industrial automation standards.
• Simple and intuitive user interface provides quick access to all controllers and data.
• You can create your own program of cultivation using “Sequences” program – for better control of your bioprocess.
• All bioprocess data is gathered and saved as separate electronic records.
• To minimize the number of errors, different fermenter operators have four different levels of access to the control system.